http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-589380-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18d92c46f60af8af24e262b67381d7eb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57407 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57434 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57438 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57488 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1997-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2004-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d067450c5af00f8866ee015a7e570f47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dafcb9266906cf0354453b840f292a9d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbd25001e4802f9f912f5ade7b4f5f4b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_421eab29ef5ef693af66e1769e5d8f6c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91fc3d87c3822e77ac1bfbd2839e109f |
| publicationDate | 2004-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | TW-589380-B |
| titleOfInvention | A method for enriching rare cells by using density gradient and negative selection |
| abstract | The present invention provides for enriching rare cells in a fluid comprising the rare cells and non-rare cells. Examples of rare cells enriched include prostate cancer cells. A method for enriching cancer cells in a bodily fluid sample comprising cancer cells and non-rare cells comprising: (a) obtaining the sample comprising cancer cells and non-rare cells; (b) subjecting the sample to multiple density gradient separation comprising a first density gradient and a second density gradient, wherein the second density gradient is greater than the first density gradient, and producing a first fluid comprising an increased concentration of cancer cells of a first density, and a second fluid comprising an increased concentration of cancer cells of a second density, wherein the second density is greater than the first density; wherein subjecting the sample to multiple density gradient separation includes producing a plasma layer, a first interface layer, a first gradient layer, a second interface layer, a second gradient layer, and a cell pellet; and comprising using at least one density gradient medium having a density of no less than about 1.06 g/ml; wherein producing the first fluid includes combining the first interface layer and the first gradient layer and forming a first suspension; and wherein producing the second fluid includes combining the second interface layer and the second gradient layer and forming a second suspension; (c) subjecting said second fluid comprising the second suspension to a binding agent that binds non-rare cells; (d) removing the bound non-rare cells from the second fluid to provide a second fluid enriched with the greater density cancer cells, and (e) preparing a fluid enriched with the cancer cells of the first density and the cancer cells of the greater density by combining the cancer cells of the first density from the first fluid and the greater density cancer cells from the second fluid enriched with the greater density cancer cells, wherein the method comprises increasing by at least about 500-fold the concentration of the cancer cells compared to the concentration of the cancer cells to the non-rare cells in the fluid sample. |
| priorityDate | 1996-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 178.