http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-434255-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da54f7ebbd2d9d8a82f92aca4d1b4e48 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-5421 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 1996-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2001-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2173f310cf55a56c36c4c8b5e50c0a3d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c804cc8044c6e9e661c71f695e53380a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff8adc84e37e4aba969fbccf1356affd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4eebd26fd34e838dfe9b037e86879b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f65c46fe8e71e3623e03eca930b76a5c |
publicationDate | 2001-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | TW-434255-B |
titleOfInvention | Process for the purification of recombinant human interleukin-8 |
abstract | Process for the purification of interleukin-8, characterised in that (a) firstly cells are lysed in buffered solution and (b) the resulting lysate is treated by means of mole-cular weight exclusion by repeated cross-flow ultrafiltration in such a manner that (i) in the first filtration step interleukin-8 is separated from larger accompanying components and (ii) in the second filtration step is separated from smaller components, (c) the buffered filtrate from process step (b) is subjected to high-resolution purification by means of cation-exchange chromatography, wherein the highly positively charged surface of the inter-leukin-8 molecule is used in a form such that the application and elution pH value is selected to be sufficiently high that the interleukin-8 can just still bind, then (d) the buffered solution of the interleukin-8 eluate from process step (c) is exchanged by gel filtration, dialysis or ultrafiltration and finally (e) the interleukin-8 solution obtained via process step (d) is lyophilised. |
priorityDate | 1995-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 100.