http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-381178-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8ebfc8d66ac1303ed1e3e999d65aa236 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
filingDate | 1994-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2000-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c53a894935d48b9bdcc3f90e79f8d9d3 |
publicationDate | 2000-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | TW-381178-B |
titleOfInvention | Compositions and methods for protein structural determinations |
abstract | A method for determining three-dimensional structural information of a protein involves producing the protein in a form substantially labeled with C13 or N15 or both or substantially labeled with N15 and C13 and partially labeled with H2 and subjecting the protein to nuclear magnetic resonance spectroscopic analysis. The isotopically labeled protein is produced by a method which involves producing a substantially labeled microbial protein hydrolysate, subjecting the protein hydrolysate to cation exchange chromatography to produce a partially purified labeled amino acid mixture, subjecting the partially purified labeled amino acid mixture to anion exchange chromatography to produce a purified labeled amino acid mixture and supplementing the purified labeled amino acid mixture with isotopically labeled cysteine and optionally with isotopically labeled glutamine and asparagine. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113125594-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113125594-B |
priorityDate | 1993-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 277.