| abstract |
The invention discloses a CD3 target antibody, bispecific antibody and use thereof. The antibody that targets CD3 comprises a light chain variable region (VL) and a heavy chain variable region (VH), and the VL is the amino acid sequence shown in SEQ ID NO: 56 or a mutation thereof; VH has a mutation in the amino acid sequence shown in SEQ ID NO: 42, the mutation is selected from one or more of the following positions: position 30, position 73, position 76, position 78, position 93 And the amino acid residue at position 94. The bispecific antibody includes a first protein functional region and a second protein functional region, wherein the first protein functional region includes the antibody that targets CD3 as described above. The target CD3 antibody of the present invention reduces the toxicity caused by cytokine release syndrome, and the bispecific antibody prepared therefrom is stable and has T cell binding ability, which reduces the difficulty of production. |