| abstract |
Provide effective methods for preparing polypeptides containing unnatural amino acids, methods for introducing unnatural amino acids into polypeptides, methods for preparing tRNA combined with unnatural amino acids, unnatural amino acid introduction systems, or materials used in such materials.nA method for preparing a polypeptide containing an unnatural amino acid, which includes a process of contacting PylRS of an organism of Methanomassiliicoccales or Thermoplasmatales with an unnatural amino acid, and includes an introduction process selected from the following processes (a) and (b), process ( a): Compared with the case of using a cell-free protein synthesis system using PylRS (MmPylRS) of Methanosarcina mazei, unnatural amino acids are introduced into the polypeptide with higher efficiency; step (b): and the use of Compared with the case of the E. coli protein synthesis system with the vector of the PylRS gene controlled by the glmS promoter, and the case of the E. coli protein synthesis system using the vector with the PylRS gene controlled by the glnS promoter, Introduce unnatural amino acids to polypeptides with higher efficiency. |