Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_872dd7cd609eb2a937dcfd303d521798 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2878 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28 |
filingDate |
2019-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fccf81fc4202078a790c275d4e65ecd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe6455892e2ff87c35ddfbffe522d0a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1160636c3c4a1b38189cca9cfcbfd8d4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f3f465b6c81cc15fc49728253137065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d408b64649de07c0d3cb100b44df1f6b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_acefbc7af0c81fbc00e103d7ff4e5b12 |
publicationDate |
2020-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
TW-202003555-A |
titleOfInvention |
Methods for purifying recombinant polypeptides |
abstract |
The present invention relates to a method of purifying a recombinant polypeptide from a Host Cell Protein (HCP), wherein the amino acid sequence of the recombinant polypeptide comprises a cathepsin L cleavage site, DKTHTCPP (SEQ ID NO:50); the method comprising: (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer comprising caprylate and arginine; and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support. A method of purifying an anti-OX40 antigen binding polypeptide from a Host Cell Protein (HCP) is also provided. |
priorityDate |
2018-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |