http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-201932605-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b3cf90334d36b2e5b6c416d8ff5554e1 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-331 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-501 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-683 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2018-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_453e8cd0db9099a8683436ecaeb00123 |
| publicationDate | 2019-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | TW-201932605-A |
| titleOfInvention | DNA methylation analysis method using a next-generation sequencer and concentration method of a specific DNA fragment group |
| abstract | The DNA methylation analysis method provided by the present invention comprises the following steps: (1) utilizing a cytosine containing methylated cytosine or possibly methylated in the recognition sequence, and the above recognition site is affected by methylation a restriction enzyme, a step of digesting the DNA of the analysis target; (2) a step of treating and linking the DNA fragment mixture obtained in the above step (1) with a ligase; (3) determining the DNA structure obtained in the above step (2) a step of base sequence of each DNA construct contained in the mixture; and (4) for each base sequence message obtained in the above step (3), by identifying the respective portions of the restriction enzyme and the periphery thereof The base sequence is compared with a known genomic sequence, and it is determined that each of the recognition sites is a recognition site that is not cleaved by the restriction enzyme, or an identification site that is ligated by the restriction enzyme and then recombined by the ligase. The step of determining the methylation status of each recognition site is determined accordingly. |
| priorityDate | 2017-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.