| abstract |
The present invention provides a humanized anti-Globo H antibody or its scFv or Fab fragment, which comprises a heavy chain variable domain having three complementary regions consisting of HCDR1, HCDR2 and HCDR3, and three comprising LCDR1, LCDR2 and LCDR3. The light chain variable domain of the complementary region, where the sequence of HCDR1 is GYISSDQILN (SEQ ID NO: 4), the sequence of HCDR2 is RIYPVTGVTQYXHKFVG (SEQ ID NO: 5, where X is any amino acid), and the sequence of HCDR3 is GETFDS (SEQ ID NO: 6), where the sequence of LCDR1 is KSNQNLLX'SGNRRYZLV (SEQ ID NO: 7, where X 'is F, Y, or W, and Z is C, G, S, or T), and the sequence of LCDR2 is WASDRSF (SEQ ID NO: 8), and the sequence of LCDR3 is QQHLDIPYT (SEQ ID NO: 9). |