| abstract |
The present invention provides an analysis system that can perform an operation on an analyte that can be combined with multiple reagents before being introduced into a flow cell. The instrument may include a volume into which the reagents to be combined with the analyte are aspirated one by one. The volume may be formed as a serpentine channel in a valve manifold associated with a pipette for aspirating the reagents. The reagents can then be mixed by circulating a pump to move the reagents within the mixing volume or channel. To this end, the reagents can be aspirated into the volume or channel from a receiver, sprayed back into the receiver, and the process can be repeatedly performed to enhance mixing. |