| abstract |
The invention provides a VWF fragment comprising the D ′ domain and D3 domain of VWF, a chimeric protein comprising the VWF fragment and a heterologous portion, or a chimeric protein comprising the VWF fragment and a FVIII protein, and a method for using the same. A polypeptide chain comprising a VWF fragment of the present invention binds or associates with a polypeptide chain comprising a FVIII protein and the polypeptide chain comprising the VWF fragment can prevent or inhibit endogenous VWF from binding to the FVIII protein. By preventing or inhibiting endogenous VWF from binding to FVIII, the VWF fragment can induce an increase in the half-life of the FVIII protein, where the binding is a half-life limiting factor for FVIII. The invention also includes nucleotides, vectors, host cells, methods using the VWF fragment or the chimeric proteins. |