| abstract |
The invention provides a method for preparing an anticancer component triterpenoid in a sauerkraut, which is a supercritical CO2 fluid and an ethanol 1:0.1-0.2 under an operating condition of a pressure of 2000-4000 psi and a temperature of 40-60 ° C. (v/v) mixed to saturation, and introduced into the extraction tank containing 1-2 kg of Antrodia camphorata at a flow rate of 6-9 L/hr for extraction, and then extracted into a chromatography column for separation, which can be separated at the top of the chromatography column. The high-concentration triterpenoid active ingredient was obtained, and the prepared triterpenoids were tested for anticancer effects by cytotoxicity test, cell type change analysis, cell cycle and apoptosis detection, cell crawling transfer and the like. |