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filingDate 2016-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2017-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber TW-201713772-A
titleOfInvention Method of making recombinant protein
abstract The present invention relates to a method of producing a recombinant protein in a host cell comprising the addition of polyethyleneimine (PEI) during cell culture. Addition of PEI as a fermentation enhancer to the cell culture can result in a decrease in the viscosity of the cell culture, and/or an increase in the extracellular concentration of the recombinant protein, and/or a period of cell culture to the point of harvest or protein recovery. cut back.
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