| abstract |
The present invention relates to a virus particle as an immunogen against enterovirus infection and a method of producing the same. In particular, the invention features the use of human embryonic kidney 293 (HEK293) cells to produce enterovirus type A viral particles, in particular, Croxavirus A6 (CVA6) particles, or Croxvirus A10 (CVA10). The granules, or both, and other optional enterovirus type A virus particles, such as Crosgi virus A16 (CVA16), and/or enterovirus A71 (EV71). Surprisingly, the viral particles harvested in HEK293 cells are quite high and can effectively elicit an immune response against enterovirus infection, particularly for CVA6 and CVA10. The invention also relates to a human immunological composition against enterovirus infection, comprising a viral particle as described herein, and a method of preventing an enterovirus infection or a disease caused thereby, in particular, a hand-foot-and-mouth disease (Hand- Food-Mouth disease; HFMD), by administering the immune composition to an individual in need thereof. |