| abstract |
The present invention provides for the use of charge repulsion to achieve the promotion of separation and/or binding of the Fc region, the variation of the amino acid forming the interface of the Fc region, and/or the stability of the heavy chain CH3 region to be unstable. A method in which a heteropolypeptide of a plurality of peptides introduced into a heavy chain CH3 region is introduced into a heavy chain CH3 region and incubated under reducing conditions to efficiently and stably produce a heteromultimer. |