| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e04a9c30b01a6909696dc92e883f241c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_886c57ba4cb9636082990daa6d762cb5 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2015-8518 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0682
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-1018 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2010-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_705cd69e603f933dc1654c1c7e28474b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bf8b7f60aab7196bcea67dc5b562b66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0aec26fc278b99f152cdc98a5720ad0a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6dfeb1a0c6cc57593136411d7c813ab |
| publicationDate |
2015-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
TW-201546281-A |
| titleOfInvention |
Protein production method |
| abstract |
The present invention relates to a method for producing a target protein and a mammalian cell expressing a suspension of a target protein, which is produced by a transposon at both ends of a gene fragment comprising a DNA encoding a target protein and a selectable marker gene. The sequence of the protein expression vector is introduced into the suspended mammalian cell, and the gene fragment inserted between the pair of transposon sequences is incorporated into the chromosome of the mammalian cell to obtain a mammalian cell expressing the target protein. The mammalian cells are subjected to suspension culture to produce the target protein. |
| priorityDate |
2009-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |