| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d4c6a2eb66d07fad39d708e5a36c9350 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-072
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-052
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2207-15 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0635
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0278
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate |
2014-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a25f5b3062bc576511e63e651794942e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2f3a866735a501b1cb92f49b8ca33c4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4ec9e6f69def76312ebb06f432e18e8 |
| publicationDate |
2015-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
TW-201542816-A |
| titleOfInvention |
Methods, cells and organisms |
| abstract |
The present invention relates to the introduction of one or more known sizes of desired insertions and/or deletions to one or more predetermined positions on a nucleic acid (e.g., in a cell or organism genome). The inventors have developed that the isolated DNA fragments of a predetermined size can be inserted into a predetermined position on the gene body in a sequential manner or by precise insertion, or the separation of the predetermined size can be performed at a precise position. This technique is based on the observation that single strand breaks of DNA tend to be repaired via HDR pathways, and the present invention can reduce the chance of indels (as produced by NHEJ) and thus be more efficient than prior techniques. The invention also provides sequential insertion and/or deletion using single or double strand DNA cutting techniques. |
| priorityDate |
2013-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |