| abstract |
Instead of using animal serum or feeder cells, a technique for modulating NK cells having high cell-damaging activity by high-purity by hematopoietic precursor cells has been developed.nThe method for amplifying NK cells comprises the steps of amplifying a hematopoietic progenitor cell using a single culture condition using a medium containing IL-15, SCF, IL-7 and Flt3L, and culturing using a medium containing IL-2. The condition is such that the cell differentiation obtained in the aforementioned amplification step is induced into NK cells. And a pharmaceutical composition comprising NK cells prepared by the NK cell expansion method of the present invention. The pharmaceutical composition of the present invention is useful for the treatment of infectious diseases and/or cancer. |