| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8bd7a45a343acd10e3f5d8775dda2157 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-395
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 |
| filingDate |
2014-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1b5b4144f14bc107a2a8b5296e86dba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6bb1e3bddbc09050123dbc14d2722e61 |
| publicationDate |
2014-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
TW-201446796-A |
| titleOfInvention |
In situ affinity maturation of antibodies |
| abstract |
The present invention relates to a method of mimicking a germinal center response to generate antibodies having different binding properties or higher affinities directly in a fusion tumor. To facilitate the rapid affinity maturation of antibodies, a controllable activation-induced cytidine deaminase (AID) expression system was developed which induces somatic high frequency mutations in fusion tumors. By affinity-activated cell sorting techniques, high-affinity antibodies can be selected from fusion tumors that preferentially bind to the fluorescently labeled antigen. The present invention also relates to a novel method of producing a fusion tumor having a tunable antibody affinity by generating a myeloma fusion partner having a controllable AID expression. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-I639699-B |
| priorityDate |
2013-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |