http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-201444975-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2014-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d39e5dd07e2eec8351d0451c0b619fa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_581138f9e83db2f7e0259de8dbabc41c |
| publicationDate | 2014-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | TW-201444975-A |
| titleOfInvention | Use of amino acid auxotrophic reconstituted prokaryotic strain for recombinant production of polypeptide |
| abstract | This document reports a method of producing a polypeptide in a prokaryotic cell comprising culturing a prokaryotic cell comprising one or more nucleic acids encoding the polypeptide in a chemically defined minimal growth medium and periplasmic or self-derived from the prokaryotic cell or the prokaryotic cell The step of recovering the polypeptide, wherein the prokaryotic cell line is an amino acid auxotrophic regenerative prokaryotic cell, wherein the amino acid auxotrophic prokaryotic cell is compared to the non-reconstituted prokaryotic cell under the same culture condition and Growth in the same growth medium requires the addition of less amino acid to the growth medium, and wherein the chemically defined minimum growth medium does not contain an amine group corresponding to the auxotrophy that has been restored in the auxotrophic reconstituted prokaryotic cell acid. |
| priorityDate | 2013-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 369.