http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-201331368-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_75b765b53e6651e9863a3a35e4c1e164 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-04 |
| filingDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29c37f558d5f39193031bbe2c40b0ad8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e836417133f06635114522db3996425 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a98233880fc12edeac68519ef6c00dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fbd8f496c4cc2ddcd48db4993cb6aa1 |
| publicationDate | 2013-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | TW-201331368-A |
| titleOfInvention | Method for purifying recombinant Helicobacter pylori neutrophil stimulating protein (HP-NAP) by single step |
| abstract | The invention discloses a single-step purification method for recombinant Helicobacter pylori neutrophil activating protein (HP-NAP), the method comprising the steps of: providing a sample to be purified containing HP-NAP, wherein the sample to be purified is taken from a sample a protein expression system capable of expressing recombinant HP-NAP; the sample to be purified containing recombinant HP-NAP is purified by a diethylaminoethyl (DEAE) ion exchange resin at a predetermined pH interval; and collected via the above And a flow through fraction and a wash fraction, wherein the effluent and the cleaning solution comprise recombinant HP-NAP of a predetermined purity. The present invention utilizes a high affinity DEAE Sephadex ion exchange resin to purify the recombinant HP-NAP of the water-soluble protein in a single step, thereby purifying the recombinant HP-NAP with low endotoxin residue from the Bacillus subtilis expression system, and purifying it. The recombinant HP-NAP has a typical alpha-helix secondary structure and activates neutrophils to produce reactive oxygen species. |
| priorityDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.