http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-201326398-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8983a064d5a6c9324e4285203084e02a |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2011-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53aa0d621bcfb00e3a63445d90135f23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21bdd3878ed7fdfd7511949eb3d05571 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0a5dd0506b29289a35714b83cee7d30 |
publicationDate | 2013-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | TW-201326398-A |
titleOfInvention | Method and primer set for detecting mutation |
abstract | A method based on polymerase chain reaction (PCR) is disclosed herein, which can be used to detect whether an insertion/deletion variation occurs in a selected region of a target gene in a sample (compared to a reference sequence) ). The target gene has a template strand and a crypto strand. This method uses a primer set containing a suppression primer and a forward primer. The suppressor primer used has a partial sequence overlap with the forward and the sub-sequences, but has a different melting point, and the inhibition of the 3' end of the primer can prevent the inhibition of the primer from prolonging during the PCR process. Thus, under specific PCR conditions, detection of a PCR product means that an insertion/deletion mutation has occurred in the selected region, and no detection of the PCR product means that no insertion/deletion mutation has occurred in the selected region. . |
priorityDate | 2011-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.