http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-201229511-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fc06cad2b58bbfcc30e3c96a6eb6c926 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-7105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D495-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-544 |
| filingDate | 2011-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5bb0e305204d22e628f9eb194f01bb66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f992fabca1f2911602298fbedff166ba |
| publicationDate | 2012-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | TW-201229511-A |
| titleOfInvention | Method for conjugating nucleic acid and small molecular |
| abstract | The original aim of this research was to develop a new phosphoramidation reaction suitable for nucleic acidand small organic compound conjugations. An amine-containing biotin derivative (11) was synthesized and used to systematically optimize a previously ineffective and inefficient phosphoramidation reaction method. The improved phosphoramidation reaction increased yields of nucleic acid-11 conjugates up to 80% after 3-h reactions. We demonstrated any nucleic acids with a terminal phosphate group were suitable reactants in the new phosphoramidation reactions to conjugate with nucleophile molecules such as derivatives of biotin and fluorescein, proteins and peptides. Polymerase chain reaction (PCR) studies of 11- or fluorescein-tagged DNA primers prepared by the phosphoramidation reactions indicated base-pairing characteristics of the nucleic acidconjugates were substained. This study developed a facile phosphoramidation reaction approach to prepare nucleic acidconjugates with good yields which have implications for broad biomedical applications. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-I471331-B |
| priorityDate | 2011-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 153.