http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-201219571-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_119826cf2ffee645958c8b656d59c71e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 2010-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_654115056e85512d3ad23cbaad10d673 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a14cf1cb6e9b770db96c6585a617905a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07c0df8de418122fa655854ca1f8cce6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_621aa491db1bf67bdd861679473f929c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c08fcc482bc1e1c29d3ee4caf4d60329 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_244250f698972be808d0e9e25ab1f42c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4031bb644ffb1fd1b44445efd4463533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b488ebd79ada72208ddb99f3312bb8ef |
| publicationDate | 2012-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | TW-201219571-A |
| titleOfInvention | Method for recombinant botulinum neurotoxin a vaccine production by baculovirus expression system and vaccine thereof |
| abstract | Disclosed is a method for recombinant botulinum neurotoxin A vaccine production by baculovirus expression system, comprising the steps of: (a) providing a first vector and a second vector and a reporter protein; (b) cotransfecing the first vector and second vector to a cell for recombination; (c) screening the recombinant baculovirus by the reporter protein; and (d) infecting insect cells with the recombinant baculovirus and producing BoNT/A vaccine. The recombinant BoNT/A HC produced by the present invention has higher solubility. The discosed expression system has a higher yield without changing the gene codon and would not produce inclusion body and endotoxin. In the future, the expression system has a great potential to be a novel recombinant botulinum neurotoxin A vaccine production plateform. |
| priorityDate | 2010-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.