http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-201121426-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6307733d0b7b0574c82cb0051e06cc0c |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01P7-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N63-02 |
| filingDate | 2009-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_000353d8b25dbdf9021c880bc97a5482 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e4eaa99e2d259906f150fdc212f6a88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e743801ca9f798230119d6075dcdad98 |
| publicationDate | 2011-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | TW-201121426-A |
| titleOfInvention | Insecticidal gene and protein from pseudomonas sp. TKU015, production method and application thereof |
| abstract | The present invention relates to insecticidal gene and protein from pseudomonas sp. TKU015, production method and application thereof. The production method includes the following steps: (1) culturing Pseudomonas sp. TKU015 under a condition that is optimally suitable for nattokinase production; (2) recovering the culture supernatant to isolate nattokinase solution; (3) performing molecular biological experiments on the amino acid sequence fragments obtained by adopting liquid chromatography-tandem mass spectroscopy(LC-MS/MS) analysis on the nattokinase solution to find out related genes; (4) designing suitable primers to find out insecticidal protein gene sequence fragments in pseudomonas sp. TKU015 by polymerase chain reaction(PCR); (5) using molecular biological techniques to further obtain a full-length gene of insecticidal protein; (6) transferring the insecticidal protein gene to Escherichia Coli host for over-expression; and (7) using a purification system on vectors to isolate and purify insecticidal protein for obtaining a purified insecticidal protein. The present invention also provides insecticidal application of insecticidal protein from pseudomonas sp. TKU015. |
| priorityDate | 2009-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.