abstract |
A mammal dedicated cell lin, named as (HepG2/NF-kB/Luc/sr39tk)1_18, is provided. Firstly, a pNF-kB/Luc HepG2 cell line is isolated. Then, the dedicated cell line sensitive to TPA and MTX is generated by experimental selection. Then, it is transferred a plasmid construct carrying pNF-kB/sr39tk genome into the dedicated cell line according to superfect protocol. Finally, G418 and ZEOCIN isolations are applied. The cell line of (HepG2/NF-kB/Luc/sr39tk)1_18 can be applied to the drug screen for liver cancer and examine these cells by bioluminescence imaging system and nuclear medicine image. |