http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-201005098-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b1aed73250728e8ba8636039a841bb47 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-32 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2008-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5d519f282c96f2306db633b51f55ae3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4e4e29fd430c4ae2033fbf749f3cc2b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67a6c5330b9c9a442bacb7ab5f61b8b4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e81da34b6624237726dca71063786ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e775d263d1a9de0f8af087f9a125876 |
| publicationDate | 2010-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | TW-201005098-A |
| titleOfInvention | Active mycobacterium tuberculosis chip detection method |
| abstract | An active mycobacterium tuberculosis chip detection method is disclosed. A specific target gene cluster selected from a regions-of-difference in mycobacterium tuberculosis is taken as a specific detection target for mycobacterium tuberculosis, and a nylon membrane then is selected. The target gene cluster containing 14 target genes, an internal control, and a blank control are repeatedly spotted on the nylon membrane by utilizing an automatic punch machine. After performing a crosslink, the target gene cluster is allocated on the nylon membrane to form a gene array nylon membrane, thereby completing a preparation for prototype mycobacterium tuberculosis detection chip. Afterward sputum sample of a phthisis patient is collected. Messenger ribonucleic acid (RNA) in the sputum sample then is extracted. Sufficient required complementary deoxyribonucleic acid (cDNA) is synthesized by reverse transcription experiment and is marked to form a marker for probe. Afterward the foregoing prototype mycobacterium tuberculosis detection chip and the marker are performed with probes labeling, hybridization, and post-hybridization. Hybridized prototype mycobacterium tuberculosis detection chip and the marker are performed with color development. Finally, an image result, which has been performed with the color development, is automatically analyzed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102013109065-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014057572-A |
| priorityDate | 2008-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.