http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-200904458-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a7c8179580a8082f8d9447ca5230ca5 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K36-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-28 |
filingDate | 2007-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62a7ce9014089e2e047f5487f4525202 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_25984cb44bdc7ba21d7850921860d445 |
publicationDate | 2009-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | TW-200904458-A |
titleOfInvention | Method of producing N-acetyl-d-neuraminic acid and application thereof |
abstract | Genes of Anabaena sp. CH1 N-acetyl-d-glucosamine 2-epimerase (bage) and E. coli NeuAc lyase were cloned and, wholly or individually, expressed in E. coli. The bage gene has a length of 1167bp. The molecular mass of the transformed protein was determined to be 43 kDa. A stirred glass vessel containing transformed E. coli cells expressing bage gene from Anabaena sp. CH1 and NeuAc lyase gene separately is used for the conversion of GlcNAc and pyruvate to NeuAc. A maximal productivity of 10.2 g NeuAc l/L h with 33.3% conversion yield from GlcNAc could be obtained when the concentrations of GlcNAc and pyruvate are both 1.2M. The recombinant E. coli cells can be reused for more than eight cycles with a productivity higher than 80%. This new process of using recombinant whole cells to produce NeuAc has advantages of high productivity and high recycling use of enzyme, and the necessity of using ATP can be omitted. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101603023-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103060301-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103060302-A |
priorityDate | 2007-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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