http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TN-2015000059-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cd49bc6f406f006bbad6195ba2092496 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N63-02 |
| filingDate | 2015-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9018690d94acd1b18a449aa3773cd96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c261a8964de89ab51f6037e9d60adfdd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9f170d9d6304ee015f1359dd3debff8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8884c4a165e47284cd0819e33003e605 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5d1054abe078f9036d52d574b491377 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8a20be05fb109b65520b24836909eb0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73d9e9633e4126fe6879767ca9ed067d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4dcd454c410234f7c04c5c7d8883ad9c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_359f2575358cddea400cfb704226f198 |
| publicationDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | TN-2015000059-A1 |
| titleOfInvention | Lipopeptides of Bacillus mojavensis A2 l: Structural characterization, functional properties and application in the solubilization of diesel |
| abstract | The present invention is based on the use of a novel strain producing a novel lipopeptide mixture. The invention describes the structural characterization of lipopeptides of B. mojavensis A21, the study of certain biological and functional properties as well as their potential in the solubilization of diesel. The diversity of lipopeptides produced by the A2 strain has been studied by two approaches. The first one, based on amplification via specific primers, revealed the presence of fengycin and surfactin synthesis genes. The second approach, based on the MALDI-ToF MS technique, carried out directly on the cells of the A2 strain, confirmed the presence of different isoforms of surfactin and fengycin. Indeed, this analysis revealed the presence of homologues of Cl3, Cl4 and Cl5 of surfactin or homologues of C 12, C 13 and C 14 of pumilacidine. Different isoforms of fengycins such as fengycin A C15, A C16 / B C14, A C1 7 / B C15 and A C18 / B C16 have also been demonstrated. The production of lipopeptides was confirmed by culture of the strain on Landy medium and analysis of the different families by HPLC. The strain produces a mixture of lipopeptides containing 254.39 mg / l fengycin and 89.13 mg / l surfactin. In this patent the antimicrobial potency and antioxidant activity of lipopeptides of A21 were investigated. Lipopeptides A2 have a broad spectrum of antimicrobial activity against Gram-positive and Gram-negative bacteria as well as an interesting antifungal potential. In addition, they exert a strong in vitro antioxidant activity evaluated by means of the DPPH free radical inhibition test, the B carotene bleaching test and the lipid peroxidation inhibition test. It has also been shown that A21 lipopeptides have excellent emulsifying power with respect to several couples of immiscible liquids (water / hydrocarbon). In addition, lipopeptides are stable under extreme conditions of temperature and pH. Interestingly, it has been shown that, thanks to their interfacial properties, A21 lipopeptides have efficiencies comparable to, or even superior to, their chemical counterparts (SDS and Tween 80) in increasing the apparent solubility of diesel. |
| priorityDate | 2015-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.