| abstract |
Usage: in the field of medicine, in particular when receiving drugs for the treatment of thermal burns, trophic ulcers, infected wounds. The essence of the invention: the immobilization of the enzyme preparation is carried out by processing 2% chitosan gel with 2% glutaraldehyde solution in phosphate buffer at pH 7.5, taken in a 1: 1 ratio, maintained at a temperature of 18-20 ° for 30 minutes, after which an extract of Kamchatka crab is passed through it, containing a complex of enzymes: DNA-azu 100-120 u / mg protein, RNA-azu 100-120 u / mg, alkaline phosphatase 0.05-0.07 u / mg, phosphodiesterase 1 Type 0.05 - 0.06 U / mg, protease 120 U / mg, previously dissolved in phosphate buffer pH 7.5, with against chitosan: Extract 1: 2.5 - 5 (by weight). The method allows to increase the therapeutic effect of the tool. |