http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1752769-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_73ebc6f54886e4b4f4d4497adcd307f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3aa1e6bdceb4b113c3399f96ec9800f9 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-41 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-14 |
| filingDate | 1989-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1992-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82844f8154354c0976860239c334b2c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6bbf7c5bb8a92c32c6c7624b35075d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f3116a24b5c24b50ef1f54ec6148356 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf0a70b3a35e817f0bb52b05b3db3cba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d2f407d521363e852ae68868801e2ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f233e8ecff0cb0878479049956f8e954 |
| publicationDate | 1992-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1752769-A1 |
| titleOfInvention | Method of restriction endonuclease preparation, which is able to recognize and to cleave nucleotide sequence g-t-c-g-a-c |
| abstract | Uses: biotechnologists, gene engineers. SUMMARY OF THE INVENTION: The method involves using as a Rhizobium trifolii BNI-ISXM B-194 producer strain containing only one restriction enzyme, and using a combination of cell fractionation methods in the two-phase system for purification of the enzyme in a two-phase system 7% polyethylene glycol - 2% - dextran in the presence of 0.7 M NaCI and chromatography on phosphocellulose P-11 and heparin-Sepharose with elution with a solution of NaCI in a concentration of 0.5-0.7 M, which allows a 6-8-fold increase in the yield of the target enzyme and thus reduce production costs . SO with |
| priorityDate | 1989-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.