http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1726511-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_790c282d8b08d47188a3937885907306 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 |
| filingDate | 1990-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1992-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32f6f342c85c6f6618ffa8c047676f16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5a311bb2adb26c70bc368ecd9ea5b59 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_282db4aa908488e7da4bb982572711a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08adc7ba945d242bc4e3d3571b311a43 |
| publicationDate | 1992-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1726511-A1 |
| titleOfInvention | Strain of hybridous cultured cell mus musculus l , used for preparation of monoclonal antibodies to the general antigenic determinant of human and swine insulin |
| abstract | Usage: Mus musculus L. BCKK (P) No. 420 hybrid cultured cell strain producing monoclonal antibodies (MCA) to common antigenic determinants of human and porcine insulin, used for quantitative determination of insulin using immunoassay (ELISA) or immunoblotting . Summary of the Invention: Obtaining an ICA with an increased insulin affinity constant. The strain is obtained by hybridization of splenocytes of Balb / c mice with Sp2 / 0 myeloma cells. Culture media: D MEM with 10% SEC. Passage scheme: once in 4 to 5 days in vitro. In the form of ascites, the cells are intertwined for 8-10 passages. The concentration of ICA in the culture fluid on the 7th day of cultivation was 40 µg / ml, and in ascites - 5-8 mg / ml. Using ELISA using the obtained mAb, 30 pg hormone / ml are detected. |
| priorityDate | 1990-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 110.