http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1719432-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_81a34ea94beb57379fc5d6c37867ee7c |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate | 1990-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1992-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9578189b37adb6b1ec9995dddf41bb73 |
| publicationDate | 1992-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1719432-A1 |
| titleOfInvention | Method of virus antigen isolation |
| abstract | The invention relates to medicine and veterinary medicine, in particular to chemical methods for obtaining protein fractions of viruses, and can be used in the design of diagnostic products. The goal is to increase the yield of the target product. To carry out the process, a precipitating mixture of the following composition is added to the cell culture medium,% by volume: twin 20 7-13; 10% solution of calcium chloride 10-20; 32-40% solution of PEG (mv 4000-6000) on Hanks solution pH 7.2-7.4 the rest. The resulting mixture is incubated with constant stirring at 4 ... 10 ° C for 4-5 hours, and then centrifuged. An extraction mixture containing sodium dodecyl sulfate and potassium chloride in a 0.02% Verse solution is added to the precipitate and incubated for 1.5-2 hours. Then the extraction mixture is centrifuged and the supernatant is separated. 11 tab. |
| priorityDate | 1990-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.