http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1712412-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b5f80a30268532f580031611cb3da244 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-395 |
| filingDate | 1990-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1992-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44b0fc5b8c7db0f208e8cdf83361280c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34b8bf85c4313ee45b665b04030f1877 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_547531e0aebccf8f3e74e237a7830539 |
| publicationDate | 1992-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1712412-A1 |
| titleOfInvention | Strain of hybridous cultured mammalian cells mus musculus l - a producer of monoclonal antibody to pyrophosphatase of escherichia coli |
| abstract | The invention relates to biotechnology and can be used to create an enzyme immunoassay based on the pyrophosphatase-anti-pyrophosphatase complex. The aim of the invention is to obtain a hybrid strain of cultured mouse cells producing monoclonal antibodies (MAb) to Eseherihia colt pyrophosphatase. The strain is obtained by hybridization of splenocytes of B1L / c mice immunized with the E. coli pyrophos phatase preparation. with myeloma cells. P3x63-Ad8.653 mice. The cells are cultured in Schom medium (Dulbecco's modified Iskovo medium) with the addition of 10% fetal bovine serum at 37 ° C in an atmosphere of 5% COa. The strain is designated PFB1 and deposited under the number of CASA (P) 4220. The strain produces a monoclonal IgCI antibody of the isotype, which binds to E. pF phosphorase. The content of MCA'V in 1 ml of culture medium and 1 ml of ascites liquid is, respectively, 11-12 µg and 4-5 mg. MCA can be used to create an enzyme immunoassay based on the pyrophosphatase-anti-pyrophosphate complex. 2 tab. • |
| priorityDate | 1990-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.