http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1711898-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_47246a5a3b260ce075562e24a69516a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_65a9d049dcf2fbd640391ea1932c5aad |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-118 |
| filingDate | 1989-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1992-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d88fdae2ef257378c3658307d1a8e019 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5875ceec97be96541ba585e7d96b6268 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cdba712762e30b01be909e2cb6281798 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e56b5be11753c523d5226ed73028316 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81768fd9793890484f6523a256ac5ab9 |
| publicationDate | 1992-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1711898-A1 |
| titleOfInvention | Method for preparation of chlamydious antigen for reaction of indirect immunofluorescence |
| abstract | The invention relates to medical microbiology and can be used for the serological diagnosis of various chlamydial diseases of humans and animals. The purpose of the invention is to increase the specific activity of the chlamydia test antigen. The goal is achieved by preparing from homogenized yolk sacs containing live chlamydia 20% suspension on medium 199, to which 0.05% formalin and 0.1% sodium azide are added at 4-6 ° C for 72 hours, after which the material is centrifuged at 500d15 min. Take the middle layer, bring it to medium with 199 medium to 5% concentration and lyophilize with 98-99.5% medium 199 and 0.5-2% sucrose. The invention relates to medicine, medical microbiology and can be used for serological diagnosis of various chlamydial diseases of humans and animals. And A known method for obtaining infectious test antigens of chlamydia from ovocultures is by centrifuging at 500d for l6 minutes a homogenized suspension of yolk sacs of infected chicken embryos. The middle layer containing chlamydia is used as a test antigen in the reaction of indirect immunofluorescence to diagnose chlamydial infections. However, the need to use a material containing a live infectious agent limits the practical use of this method. A method for obtaining chlamydia infection antigens is known from ovocultures by multiple differential centrifugation of a homogenized suspension of yolk sacs on saline, followed by inactivation of the formalin pathogen m at a final concentration of 0.25%. However, the known method does not provide sufficient quality of the obtained test antigens. Treatment of the pathogen with a high concentration of formalin significantly reduces the specific activity of chlamydial surface antigens. As C / 1. This reduces the intensity of their specific luminescence when interacting with homologous antibodies, the sensitivity of the reaction decreases. • Liquid test antigens have a limited shelf life. |
| priorityDate | 1989-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.