http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1708848-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bebf3a5d90d4fb8f370a7b8198b80706 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e1582e7f75abd8ce770cfc1945455b08 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-21 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-48 |
| filingDate | 1989-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1992-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb7dc877561b0c8c42ec92a6cf6dccca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64394ffc34bdc66ebe060b188e3729a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc693fc900e95e852c3bd7bf2c730272 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_72d5e9b3e53d056fab04d7d1f615e695 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee72e3dec95eb4a1ed1633895ca1ae77 |
| publicationDate | 1992-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1708848-A1 |
| titleOfInvention | Recombinant plasmid dna processing a9, encoding the synthesis of protein with antigenic properties of human deficiency virus of the first type, method for its construction, and the strain of bacteria escherichia coli - a producer of protein with antigenic properties of human immune deficiency virus of the first type |
| abstract | The invention relates to medicine and biotechnology. The purpose of the invention is to increase the yield of the target product. The EcoW-EsoZh fragment of the plasmid pAK25 was inserted into the plasmid pG30, containing the rag locus, which ensures stable inheritance of the hybrid plasmid pGA9, which has a size of 7.9 kb. The pGAQ plasmid has unique restriction sites: BamHI, Sail, determines resistance to ampicillin and kanamycin, non-conjugative, encodes protein synthesis, which possesses the antigenic properties of human immunodeficiency virus of the first type (HIV 1). Plasmid-pGA9 was introduced into the E. coli HB101 strain using the trans "| > &amp;ormation; a strain was obtained that provides protein synthesis in the amount of 650-750 µg / ml of protein, the N-terminus of which is represented by full-length beta galactosidase and the C-terminus is the Virusespecific sequence of the gag gene product, which has antigenic properties of HIV 1. 3 cf f-ly. (Ls |
| priorityDate | 1989-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 186.