http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1697008-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_81a34ea94beb57379fc5d6c37867ee7c |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 1989-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1991-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89fc95b3dbe3aff91c2180f1a83b7bef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a0b479d3071214cc7050767a55251a2 |
| publicationDate | 1991-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1697008-A1 |
| titleOfInvention | Method of leukocyte adhesion estimation |
| abstract | The invention relates to clinical immunology, as well as for the diagnosis of specific sensitization of leukocytes in the examined individuals. The aim of the invention is to improve the accuracy of the method. The method is carried out by incubating whole blood with a 0.9-1.1% solution of gelatin on medium 199 for 10-15 minutes, followed by isolation of white blood cells. The resulting suspension of white blood cells from the supernatant is precipitated and added to the wells in a concentration of 1 -1.5 mln / ml of droser-containing cells in medium 199, then the cells are incubated for 1.5-2 hours at pH 7.0-7.2 s after, washing and dosing. Adhesion tests are carried out on the activity of myeloperoxidase in adherent cells. To do this, a mixture of orthophenylenediamine and hydrogen peroxide is added to the wells in the ratio (98-102) 1, after which the optical density is measured, which is proportional to the number of adherent cells. The method is much more accurate. The positive effect of the method makes it possible to significantly improve the accuracy of the method and shorten its execution time. |
| priorityDate | 1989-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 18.