| abstract |
The invention relates to medicine and biology, in particular to methods for immunodiagnostics of chronic infectious diseases. The aim of the invention is to simplify the process by isolating the antigen from the immune complexes circulating in the blood. For this, borate buffer pH 8.4 in a ratio of 1:25 is sequentially added to the blood serum of the subject, polyethylene glycol with mol. 6,000 to a final concentration of 3.5%, incubated in the cold, centrifuged, the resulting precipitate is washed with 3.5% polyethylene glycol-boate buffer, applied to glass, dried in air, fixed with ethane and examined in an immunochemical reaction. |