http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1675727-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4093a51e1abe5dc09d75bc44134e6e9c |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate | 1987-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1991-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3b2144019f17ea396692f2f7ef88079 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c7638fa21c454eb780edeaf4ac27048 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b34ae5f197b8f7377786921c7dfce542 |
| publicationDate | 1991-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1675727-A1 |
| titleOfInvention | Method of preparation cell culture for electroradioautographic investigation |
| abstract | The invention relates to medicine, namely to radioautographic research methods. The goal is a more complete identification of DNA in the cell. The objective is achieved in that cells from the skin are cultured with MEM medium with 10% bovine serum at 37 ° C for 5 days. Next, labeled thymidine was added to the culture at a concentration of 10 µCi / ml. After 2 h, the culture is fixed, dehydrated and enclosed in epoxy resins. The finished epoxy disc is removed from the cup and a photoemulsion layer is applied to its lower surface, which is fixed and developed in a solution of hyposulfite and paraphenylenediamine. The disks are scanned under a microscope, the accumulated marks are cut out for electron microscopic examination. The method makes it possible to evaluate cell culture, its viability, suitability for transplantation. |
| priorityDate | 1987-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.