http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1654338-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_383d110c25d8b8c0467a7ce6103369d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_30ec91413fdc5bd16f63f1868a417d56 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 |
| filingDate | 1989-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1991-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2074dcac72bbc7c5aafc876f6f0f96fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1eb0f811d13c07cecfb7ce3373978634 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_983ec1daf9cc0959208272ceb85089e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23b08e636bdb9096b3248356cdd0d500 |
| publicationDate | 1991-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1654338-A1 |
| titleOfInvention | Nutrient medium for restoration after cryoconservation of hybridous mammalian cells, mus musculus l - producers antiinfluenza monoclonal antibodies |
| abstract | The invention relates to hybridoma technology and can be used to restore hybridomas after cryopreservation. The invention relates to hybridoma technology and can be used to restore Musnusculus L. hybridoma cells from animals after cryopreservation. . Example, To 1 liter of commercial RPMI-1640 medium was added 3.9 mg of 2-mercaptoethanol, 100 mg of gentamicin, 4.76 g of HEPES, 292 mg of L-glutamine, 200 ml of Mus musculus fetal bovine meat. Aim of the invention increase cell survival while maintaining their production capacity. To do this, to 1 liter of base medium — Fibiu-modified RPMI-1640 medium, in addition to standard additives (Hepes 4.76 g / l, L — glutamine 292.0 mg / l, 2-mercaptoethanol, 3.9 mg / l, gentamine 100.0 mg / L, 20% (blood embryo spit), components added, g / L: sodium glutamate 170-40; sodium pyruvate 50-100; A1F-3 Na 1.0-2.0; HA, T H2-2-Na, 0-4.0; Fe (NO) 0.1-0.2, PH medium;, 2 - /, 1. Using examples of six freezing-sensitive hybridomas, it has been shown that they do not harden on a known medium, but search for and restore antibodies on a modified medium. Using the example of seven other hybridomas (less sensitive), it is shown that they erupt on both versions of the media, but they form a monolayer earlier on the proposed medium. ki and get the base environment (modifications according to Fibih). This medium is effectively used for the restoration of hybridomas, which have a low sensitivity to cryogenic damage. To enhance the nutritional properties of the medium during the restoration of hybridomas, they are distinguished by their hypersensitivity to cryogenic damage, in addition to it (mg of monosodium glutamate) 170; sodium pyruvate 50; Nitric acid nitrate iron 0.1; ADP-3-sodium 1; about $ (L o JV -U with co 00 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/MD-3569-G2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9922033-A1 |
| priorityDate | 1989-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.