http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1640158-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c5ff5c641974f9657c1c7da20d6a8377 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate | 1989-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1991-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba831a60bd078189c712096188be9f0c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15a0c24726d61d9ccbb808a743662133 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e676ed26da5fb48c44fa93938547985e |
| publicationDate | 1991-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1640158-A1 |
| titleOfInvention | Strain of hybridous cultivating mammalian cells, mus musculus l, tsed for preparation of monoclonal antibodies for dna-dependent rna-polymerase of bacteriophaga t7 |
| abstract | The invention relates to hybridoma technology and virology and can be used to improve the quality of the secreted eukaryotic RNA polymerase enzyme. The aim of the invention is to obtain a hybridoma strain producing monoclonal antibodies (Mon AT) to bacteriophage T7 RNA polymerase. The strain is obtained by hybridization of silocytes of BALB / C mice immunized with the purified preparation of RNA polymerase, bacteriophage T7, with cells of the mouse myeloma of the X 63 line. AG 8.653. Cells were cultured in DMEM medium with fetal bovine serum (10%), glutamine (2 mmol), sodium pyruvate (1 mmol), 2-mercaptoethanol (0.05 mmol), penicillin / streptomycin (1000 units / ml). The frequency of passaging 2-3 times a week, sowing - a dose of 150 thousand cells per 1 ml, the multiplicity of sowing 5-10 times. The productivity of strain 10 (by ELISA) in culture medium and 3 x 10 in ascitic fluid. Mon AT, secreted by the strain, specifically interacts with the bacteriophage T7 RNA polymerase. The strain is deposited under the number BCKK (II) -3S2 D. Mon AT is classified as Jg G1. W |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115466731-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115466731-A |
| priorityDate | 1989-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.