http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1602867-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af6eb5bf72b8f7eac76ac4e5bc2811bf |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate | 1988-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1990-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da8fc5d4ea55f38633229ed6a075123e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2f6f8c046a39300ae7851bfa72e183d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f67606c612106372df9918c2890572e9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b0a694b48ec092d9dbb2849597054ce |
| publicationDate | 1990-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1602867-A1 |
| titleOfInvention | Strain of hybrid cultivable mus musculus l cellis used for producing monoclonal antibodies to human collagen |
| abstract | This invention relates to hybridoma technology and can be used in medicine and biology. The aim of the invention is to obtain a hybrid strain of cultured animal cells MUS MUSCULUS L producing monoclonal antibodies (MON AT) to human collagen type I and III. The strain is obtained by hybridization of spleen cells of a BALB / C mouse, immunized with human type I collagen, with X63 P3 / Ad 8.653 mouse myeloma cells. The strain was deposited as VSKK number (P) N163 D. The hybridoma was cultivated in the DMEM medium with bicarbonate and 20% fetal bovine serum, 2 mM L-glutamine, 2 mM pyruvate sodium, 100 µg / ml kanamycin, 5 µg / ml Fungison, 0 , 05 mM 2-mercaptoethanol at 37 ° C in an atmosphere of 5% CO 2 . The strain produces MONs of the AT class YGM, which specifically bind to human collagens type I and III. For 3-4 days of cultivation, the concentration of MOH of AT is 1-10 µg / ml in culture medium and 10-30 mg / ml in ascites fluid. |
| priorityDate | 1988-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.