http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1602866-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af6eb5bf72b8f7eac76ac4e5bc2811bf |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate | 1988-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1990-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da8fc5d4ea55f38633229ed6a075123e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b0a694b48ec092d9dbb2849597054ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2f6f8c046a39300ae7851bfa72e183d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f67606c612106372df9918c2890572e9 |
| publicationDate | 1990-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1602866-A1 |
| titleOfInvention | Strain of hybrid cultivable mus musculus l cells used for producing monoclonal antibodies to human collagen |
| abstract | This invention relates to hybridoma technology and can be used in medicine and biology. The aim of the invention is to obtain a hybrid strain of cultured animal cells MUS MUSCULUS L producing monoclonal antibodies to human type I collagen. The strain is obtained by hybridization of spleen cells of a BALB / C mouse line immunized with human IY-type collagen, with X63.P3 / Ad 8.653 mouse myeloma cells. The strain deposited under the number VSKK (P) 164D. Hybridoma is cultivated in a DMEM medium with bicarbonate and 20% fetal bovine serum, 24 mM L-glutamine, 2 mM sodium pyruvate, 100 µg / ml kanamycin, 5 µg / ml fungizone, 0.05 mM 2-mercaptoethanol at 37 ° C 5% CO 2 atmosphere. The strain produces class I dM monoclonal antibodies that specifically bind to human type I collagen. For 3-4 days of cultivation, the concentration of monoclonal antibodies is 1-10 mg / ml in the culture fluid and when cultivated in an animal in ascites - 10-30 mg / ml. |
| priorityDate | 1988-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.