http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1597728-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efe23bc297327f6cb207c8ad57315d23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec952ff5d2ae5e1edc02867000aab618 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 |
| filingDate | 1988-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1990-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f56f9e2612062c1e1f8f518a968b039 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13ecd28c279783e43e3b0cc22d22528c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e2cf29716482619e393be48868ea8cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bca944fd168743f0f612aacf15eddd92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c74b8a12dcdf1c686e692ab9c479bc52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9cd836102a555120551e0733a6c72232 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b2c1718207e70156d9e6bf8145ece03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e0901e0bb0bc09b5eace2085a88480a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a83aaebe6e43dcdf6e636d0890fcd2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d657a00ee9b6ba6ed3523ca0cb925ef7 |
| publicationDate | 1990-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1597728-A1 |
| titleOfInvention | Method of determining antibodies to virus of manъs immunodeficiency in blood |
| abstract | The invention relates to the field of medicine, specifically to the immunodiagnostics of acquired immunodeficiency syndrome, and can be used to determine the presence of antibodies to proteins of the HIV virus in the blood. The goal is to increase the sensitivity and simplify the method. In the proposed method of detecting antibodies to HIV proteins, SDS electrophoresis in a gradient 4-20% acrylamide gel is used, virus-containing material - extracts of virus-producing cells of the lymphoblastoid line NTA-4. Separated in acrylamide gel to individual protein bands, HIV antigens are transferred by electromigration to a nitrocellulose filter, viral antigens are identified and nitrocellulose filters bands corresponding to p24, p55, other 41, dr 120 proteins are cut and fragments of these bands are mounted with nitrocellulose glue on a special paper plate , forming a diagnostic strip. The active part of the Diagnostic strip, which consists of 4 fragments of nitrocellulose filters, each of which carries one HIV protein on its surface: p24, p55, others 41, others 120, is immersed in the analyzed serum, diluted 1:50 with saline. After incubation, a diagnostic strip is developed using an anti-species conjugate, similarly to the immunological development of the prototype method - Western blot. In order to increase the reliability of the assay results in the ELISA, two marker enzymes, β-lactamase from BACILLUS LICHENIFORMIS and horseradish peroxidase, are used. |
| priorityDate | 1988-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.