http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1469464-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e41596d60a235a036afd355e094fefaa http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8d05a9c67f37c5f9254bb7e8f9134b32 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-49 |
| filingDate | 1987-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1989-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1b59b316d5e4814b681a4131fd7fdb4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb34ce3fac709ceeb059c05c78cbb7b0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3193895ef3ce415e493399de8c9f56b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d44378ab905fbf04edb3c23f5cf15b81 |
| publicationDate | 1989-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1469464-A1 |
| titleOfInvention | Method of determining erythrocyte resistivity |
| abstract | The invention relates to medicine, more specifically to hematology, to laboratory methods for the extent of damage to red blood cells, intended to study the physicist-chemical state of canned red blood cells and for clinical practice in laboratory methods for evaluating the state of erythrocyte membranes in various pathologies of the blood system. The purpose of the invention is to accelerate and increase the accuracy of the method. For this, an isotonic solution of NaCl is added to a blood sample stabilized by heparin or sodium citrate, centrifuged at 3000 x 5 minutes. Isotonic p-p NaCl (dilution 100-150 / times) is added to a separate erythrocyte upset, the suspension is divided into 2 equal parts, control and experimental. The control sample is used to obtain hemolyzed erythrocytes (100% hemolysis) by adding 2% triton X-100 solution to the suspension. A test sample is obtained by adding an isotonic NaCl solution to a suspension of erythrocytes similarly to a control sample, heated 5 {n at 48–50 ° C. After temperature incubation, the red blood cell suspension is centrifuged for 5 minutes at 3000g and the supernatants are collected and recorded at their optical density at А 540 nm. Since the Triton X-100 detergent was added to the control tube, all erythrocytes were hemolyzed and the optical density of this sample was taken to correspond to 100% hemolysis (D 100%). On this basis,% hemolysis is calculated at 48-50 ° C according to the formula:% hemolysis (D48.) / D, oo A, where 4s-3o c is the optical density of the test sample; 100 is the dilution value of the samples. With a hemolysis value of 5–9%, a moderate decrease in resistance is determined, with hemolysis of more than 10%, a pronounced degree of decrease in resistance of 3pnTpoipiTOB. “2 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7981681-B2 |
| priorityDate | 1987-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.