http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1462199-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fc74faee4cb4b0432ec503c10230f6d2 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate | 1986-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1989-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee7c3963f737684e2e85e2100d28dcc1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58c3e8210f922b528ddf5de9c60ca59b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e951aa8af2733f4e50a081ef434f38f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd7c4ccddb5993d56bec566edc06624b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16ec69f9ab0c5b3fa1c34436512e1ca3 |
| publicationDate | 1989-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1462199-A1 |
| titleOfInvention | Method of detecting o-antigens of gram-negative bacteria in biological material |
| abstract | The invention relates to medicine, in particular to a method for the express diagnosis of infectious diseases caused by gram-negative bacteria (typhoid fever, salmonella disease, dysentery, etc.). The aim of the invention is to increase the sensitivity of the method by treating erythrocytes with proteolytic enzymes and formalin and treating the test sample with trilon B and alkali at 50-60 ° C. Dp this is taken a urine sample, centrifuged to remove it from suspended particles, add a solution of the sodium disodium salt a-acetic acid (trylon b), shaken, freed from low molecular weight compounds by gel chromatography on gels that separate molecules with a molecular weight of not more than 30,000 daltons, the fraction whose output volume is equal to the external volume of the gel is treated with an alkali at 50 -60, her. alkaline trap, adsorb 0-antigen at 50-55 s on erythrocytes, pretreated with a proteolytic enzyme, and then with formalin, erythrocytes adsorbing 0-antigen are revealed by their specific antisortic agglutination. The method is highly sensitive. (3 - 6 ng / mp). To receive a response, it is necessary to spend no more than 2.5 cho S (/) 4 Od OD CO |
| priorityDate | 1986-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.