http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1433980-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fdba90cf2180038389fe54d9614d0934 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate | 1987-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1988-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e43c2ef1c66e9795b5caa037f37f9000 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40a7779243997d95cb8539dac48d845a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d48d60280b85a88866fa570af09caa2 |
| publicationDate | 1988-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1433980-A1 |
| titleOfInvention | Method of producing glycerolkinase |
| abstract | This invention relates to the field of biotechnology. The purpose of the invention is to simplify the cleaning process and increase the yield of the target product. The method involves the use of affinity chromatography on an agarose sorbent with a dye-bright KKH active red immobilized dye for the isolation and purification of the enzyme. The homogenate obtained after the disintegration of the yeast biomass is subjected to heat treatment (10 min at 60 s), the coagulated protein is separated by centrifugation, chromatographed on a 4B Separate column with the active blue bright CK. Fractions containing glycerol kinase are applied to a column with DEAE-Sepharose and eluted with 0.5 M NaCl solution. 3 il. 1 tab. i (o |
| priorityDate | 1987-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 438.