http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1425542-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af330e49f27592cd189400166c09ef79 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate | 1986-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1988-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_349466c4e17745da97b0983751c0a92c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83b3611a38d57b62f4113946c2ae3faf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71b44e50b921900961d787b0c6c618f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_607abf9964280a46585ec34f0a88a11f |
| publicationDate | 1988-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1425542-A1 |
| titleOfInvention | Method of analysing growth characteristic of culture media for cell culture |
| abstract | The invention relates to biotechnology and can be used to assess the growth properties of nutrient media, as well as to assess the toxicity of their components and various nutrient media supplements. The aim of the invention is to simplify the method. The method consists in that the content of carbonyl compounds in the test medium is measured, the cells of BHK-21 are grown on this medium as test culture for 2-4 days, their number is calculated, the proliferation index of the first passage is measured and the level of carbonyl compounds is measured in supra-cellular fluid. Then, according to the derived equation for BHK-21 cells, the expected average value of the proliferation index in four D passages is found: ะจ1, .4 14.3-57.3, where P, the increase in optical density at a wavelength of 505 nm, indicating a change in concentrations Schiff bases formed by the interaction of apidegids (ketones) with 2, 4-dinntrophenylhydrazine, which characterizes the level of carbonyl compounds; LOOK- proliferation index, corresponding to the ratio of the number of cells after the first passage to the planting dose; Sh1, .4- the average value of the proliferation index in four passages. 1 hp f-ly, 1 tab. with $ u ate sd to |
| priorityDate | 1986-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.