http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1381158-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b24396c7e146a3837236af8afda1b998 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| filingDate | 1986-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1988-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40f891b1e1a7d884233b84be04866399 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c003cf64e6eb2175773e27e8abf42d75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08b4ea1e85401f5f9b6ab2a886d911a2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d31eca69322bafaec72adbd5b770781 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9f0fa8fbdd6ca997aaef2f878a1f09d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b2a1e3fa2a403c5ae52f46abe61cedb |
| publicationDate | 1988-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1381158-A1 |
| titleOfInvention | Strain of hybride cultivatable cells of mus musculus mice used for producing monoclonal antibodies to human urokinase |
| abstract | The invention relates to the field of biotechnology and can be used for the isolation, purification and determination of urokinase in biological fluids. The aim of the invention is to obtain a strain of hybrid cultured cells, argay and Miis mtisculus, producing monoclonal antibodies (MA) to human urokinase, possessing a specific antigen binding constant and interacting with two molecular forms of the enzyme. . Strain NG is obtained by hybridizing splenogts with a Balb / c line immunized with urokinase, with X63-Ag8-653 myeloma cells. It is stored in the Special Collection of Transplanted Somatic Cells of the Vertebrates of the Institute of Cytology of the Academy of Sciences of the USSR under the number VSKK (P) 34D and is characterized by the following features. Culture medium; RPMI-I640 medium with 10% fetal calf and 10% horse serum, sowing dose of 10 cells. in ml, the multiplicity of sieving 1:10 every 3-4 days, culture suspension. 1 1 is transported in the form of ascites on Balb / c mice at least 3 times. The content of MA in the culture fluid on day 4 of cultivation is 20-30 µg / ml, in mature ascites 2-3 mg / ml. MAs belong to the IgG class, (K), have a binding constant for the antigen 10 M, inhibit the activity of urokinase, interact with its two molecular forms. MAs are used to determine and purify urokinase. 1 tab. t (/ CO 00 el 00 |
| priorityDate | 1986-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.