http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1315903-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_147b970edfe8f0c2136090ddd75cf669 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 1983-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1987-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bb939fa8f6faafd3562669acbedda7e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b65e52265f2ddaa495324446d9f2f45a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2378463d920cf41cd56c92510730da92 |
| publicationDate | 1987-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1315903-A1 |
| titleOfInvention | Method of quantitative determining of radioactive steroid metabolites |
| abstract | The invention relates to the chemistry and biochemistry of natural compounds, intended for the quantitative determination of radioactive organic co. togetherness, in particular steroid hormones. The purpose of the invention is to increase the accuracy and acceleration of the method. For this, a prostate adicoma, human gland is prepared in a 0.05 mmol-l phosphate buffer, pH 7.4, C n 5 o -dihydrotestosterone at a concentration of 100 nanomolar l are incubated with the homogenate. prostate adeleomes in the presence of L-i glucose, glucose-6-phosphate,. NADH and glucose-6-phosphate dehydrogenase for 30-90 minutes at. The total sample volume is 75-100 μl. 25-50 µl of 1HHC1, steroids: 5c-androstane-Cs, 1 7 / c-diol, 5ci-androstan-3, 17/1-diol, 5c-dihydrotestosterone and lyophilized are added to the samples. 50-100 µl of benzenemethanol (1: 1) mixture is added to the residue and the samples are chromatographed in a solvent system of benzene: acetone: ethyl alcohol (9: 1: 0.5) and the plates are dried and sprayed. The mixture is: ice on acetic acid. acid - concentrated sulfuric acid - anisic aldehyde - methanol (25: 1: 3: 71), heated for 5-7 minutes to 100-1 ° C; The corresponding corresponding metabolites are cut out together with an aluminum substrate, placed in a scintillation vial, filled with scintillation mixture, radiometry of the identified zones is carried out by placing in a scintill a liquid layer of silica gel with an aluminum substrate. (L with cd with |
| priorityDate | 1983-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.