http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1298667-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ea308df827f8b4f18b2ce85c386c1777 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
filingDate | 1985-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1987-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6ed98d9a9d50c9c9e875a6084d0f942 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ef18aa7a8c00ee7b838676e2e9aaff4 |
publicationDate | 1987-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | SU-1298667-A1 |
titleOfInvention | Method of diagnosis of endometrium functional insufficiency in case of habitual miscarriage |
abstract | This invention relates to medicine, more specifically to obstetrics. The purpose of the invention is to improve the accuracy of the method. To do this, take a biopt at the endometrium on the 21-24th day of the menstrual cycle. The biopsy is repeatedly washed in physiological solution, homogenized and centrifuged for 15 minutes at 3000-8000 rpm. In the supernatant, creatine phosphine kinase (CPK) activity and protein concentration are determined. For the determination of CPK, 20 µl of the endometrium homogenate tested is taken to 525 µl of substrate containing 100 mmol of 3-standardamine buffer, pH 7.0, 35 mmol / l of creatine phosphate, 20 mmol / l of glucose, 9 mmol / l of glutadione, 10 mmol / l of acetic acid magnesium, 1 mmol / l ADP, 0.6 mmol / l NADP, 10 mmol / l AMP, 1.2 kE / l min of glucose-6-phosphate dehydrogenase, 1.2 kE / l min of hexokinase. The mixture is incubated at 5 min. Then, at 340, they measure the change in optical density in a 1.0 cm cuvette after 1.0 min for 3; -x min. The enzyme activity is calculated according to the formula A / min-3524, expressed in μmol / min-ml. The protein content was determined by the Lowry method and expressed in mg / ml. The specific activity of CPK is expressed in μmol / min-mg. If the specific activity of CPK is more than 20 µmol / min.mg, functional insufficiency of the endometrium is diagnosed. § (LU from 005 O5 |
priorityDate | 1985-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.